It was from a Katie Barker free-kick that the opnly goal came, her delivery was flicked on for Julie Lawrence to nod past the keeper. Lindsey Williams made a good save, having to change direction to stop a deflected shot.
Gloucester came at Penzance more in the second-half but Katy Barker almost grabbed a second goal but shot the wrong side of the post when running through. Amy Hemingway suffered a cracked toe as the result of a tackle but played on to the finish.
The Magpies host Larkhall Athletic this Sunday, kick-off 2pm at Penlee Park.
Penzance: Williams, - Hurworth, Bateman, B.Lawrence, - Hemingway, J.Lawrence, Mansfield, Small, Barker, - Knuckey, Hall.